Effects on Determining Mannitol Content of Myceliun of Cordyceps Sinensis by Different Culture Condition

Object: To study the effects of mannitol content and mycelium weight by different culture condition in mycelium of cordyceps sinensis. Method: Colorimetric method was used to determine mannitol content. Mycelium weight was weighted after drying. Result: The mycelium weight was maximum at 16 days of shaking with ammonium nitrate as the optimum source of nitrogen. To obtain a high mannitol content in culture and mycelium, it was necessary to have 8 days of shaking and the optimum source of nitrogen was ammonium ohloride. Conclusion: The mannitol content and mycelium weight were affected by shaking time and source of nitrogen.