Study on Improvement Effect of Triton X-100 for Individual Bacterial Colony PCR Method

The individual bacterial colony PCR method was used to screen the recombinant plasmids containing foreign genes and homologous recombinants directly.The clones were cultured and selected from E.coli DH5α,Listeria monocytogenes respectively.The methods were improved by treating PCR templates with 0.1% Triton X-100.From the agarose gel electrophorograms,it was found that the positive identification rate of screening could be increased dominantly through treatment of template by Triton X-100.This study suggested that the individual bacterial colony PCR method could be improved by treatment of PCR templates with 0.1% Triton X-100,and a system of 15 μL PCR mix would be the best choice.The special method for Gram-positive bacteria could be applied to the promotion of a variety of pathogens.