The Expression and angiogenesis Effect of VEGF gene

To study the expression and its angiogenesis effect of VEGF gene The human VEGF cDNA was cloned into eukaryotic expression vector pcD2 The recombinant plasmids were transfected into VSMC and bygene suture transferred into the adductor of rats of which the distal extreme of external iliac artery weere ligated With ELISA immunohistochemistry and alkaline phosphotase staining we investigated the in vitro and in vivo expression and its angiogenesis effect of VEGF gene The expression of VEGF gene in VSMC showed that pcD2/VEGF Was significantly higher than pcDNA3/VEGF(1103.86 59.49 vs 751.94 208.13 pg/m1/2 105 P 0.05)In vivo experi- ment also showed that the expression of VEGF gene in rats was evident Fourteen days after the initiation of gene therapy histologically identifiable capillaries were increased in VEGF transfectants compared with controls (16.47 0.33 /10 40 P 0.01).The results demonstrated that the ttansfer of VEGF gene stimulated the formation of focal microvessels and pcD 2 /VEGF was better than pcDNA3/VEGF plasmid for VEGF gene therapy