Influence of Ginkgetin Aglycone on the Expression of Lectin-like Low Density Lipoprotein Receptor-1 in Endothelial Cells

Objective: To investigate the regulating effects of ginkgetin aglycone(GA) on oxidized low density lipoprotein(ox-LDL) induced gene and protein expression of lectin-like oxidized low density lipoprotein receptor-1(LOX-1) in human umbilical vein endothelial cell line 304(ECV304).Methods: RT-PCR and SP immunohistochemistry assay were used to detect the expression of LOX-1 in endothelial cells under the induction of ox-LDL and the intervention effect of GA.Results: The expression of LOX-1 mRNA and its protein was inhibited significantly when ECV340 cells were incubated with 6.25~25 mg/L GA for 6~48 hours(P0.05),and to some extend,the inhibitory effect appeared dependant on the drug concentration and action time(P0.05).LOX-1 receptor inhibitor,polyinosinic acid showed inhibition to the expression of LOX-1 mRNA and protein induced by ox-LDL(P0.05).Conclusions: Endothelial dysfunction could be caused by ox-LDL through LOX-1,and GA can inhibit significantly the expression of ox-LDL induced LOX-1,which may be one of its anti-atherosclerosis mechanisms.