A Study of Basic Immunological Substances in Foetus Cord Blood

Objective: To study the immunity state of foetus cord blood,so as to provide theorty basis for the application of immunological substances from foetus cord blood in hematoplasma immunotherapy.Methods: Using blood routine test kits,liquid scintillation counter and flow cytometry to detect leukocyte classfication,immune globulin,T cell antigen surface marker and NK cell respectively.Results:IgA and IgE in cord blood were much less than in adult peripheral blood,but IgG was nearly the same as that in adult peripheral.G-CSF and GM-CSF in cord blood plasma were 11.37 and 15.81 times respectively of those in adult peripheral blood,while tumor necrosis factor(TNF) was a little bit lower,and heterophil granulocyte level was close to the lower limit in adult peripheral blood.Metagranulocyte and myelocyte levels were obviously lower than those in adult peripheral blood.Leukomonocyte level was about the same as that of adult.The counts of subpopulations CD3+,CD4+,CD8+ of T cell in cord blood were significantly lower than those in adult peripheral blood while those of CD34+ and CD45RA were higher.NK cell killing activity was slightly lower than that of adult blood.Conclusion: Relative hemopoiesis factors and native hematopoietic stem cell components,G-CSF and GM-CSF,are abundant in cord blood and can be widely used in clinical immunotherapy,even though most T lymphocytes in cord blood are in naive stages of development.