Study on Optimized Fermentation Medium of Acetic Acid Bacteria As1.41

[Objective] The research aimed to optimize the medium of acetic acid bacteria As1.41.[Method] Acetic acid bacteria As1.41 was taken as the experimental spawn,and the acid-base titration was adopted to measure acetic acid contents.The optimizing of the medium used in the production of acetic acid was studied by single factor and the orthogonal experiments.[Result] The best carbon and nitrogen sources were glucose and yeast extract.The best proportion of the medium contents for Asl.41 was glucose 1%,yeast extract 1%,ethanol 4%,and acetic acid 0%.Under this condition,the output of acetic acid could reach 56.6 g/L.[Conclusion] The study could provide reference for large-scale production of acetic acid fermentation.