Expression of mRNA of glucose 6 phosphatase and phosphoenolpyruvate carb-oxykinase kinase on rat models of obstruction jaundice and recannalization of biliary tract

Objective To investigate the expression and significance of mRNA of glucose-6-phosphatase(G-6-P) and phosphoenolpyruvate carboxykinase kinase(PEPCK) on rats with obstructive jaundice and recannalization of biliary tract,and to explore the molecular mechanisms of glucose metabolism affected by the obstructive jaundice.Methods Thirty healthy male Wistar rats were divided into five groups at random: group A(sham group),group B(obstructive jaundice one week group),group C(obstructive jaundice two weeks group),group D(obstructive jaundice one week and recanalization one week group),group E(obstructive jaundice two weeks and recanalization one week group).The levels of serum alanine aminotransferase(ALT),direct bilirubin(DBIL),and total bilirubin(TBIL) were measured.Morphological changes in hepatic tissues were observed under the light microscope.Expression levels of G-6-P and PEPCK mRNA in hepatic tissues were detected by RT-PCR.Results After obstruction,the levels of serum ALT,DBIL,TBIL in group B and C were increased significantly compared with group A,especially in group C.Pathological morphological has changed in hepatic tissues of group B and C,including swelling of hepatocytes,infiltration of inflammatory cells,and bile duct hyperplasia in portal area.Necroses of sheet liver cells were observed in group C which pathological change was more seriously and bile ducts and fibrous tissue proliferation were more obvious.Expression levels of mRNA of G-6-P and PEPCK in hepatic tissues of group B and C increased markedly compared with group A.Compared with group B and C,the levels of serum ALT,DBIL,TBIL were lower,expression levels of mRNA of G-6-P and PEPCK were lower,and morphological changes of liver were more normal in group D and E.Group D was less serious compared with group E in all aspects.Conclusion After the model of bile duct obstruction was constructed bile began to silting-up.With the biliary obstruction prolonged,the level of mRNA of G-6-P and PEPCK were increased in hepatic tissues time-dependently,and enhance the process of gluconeogenesis,lead to the elevation of blood sugar.However,the liver function and morphology began to recover time-dependently after bile flow restoration.The earlier remove obstruction,the faster recovery.Disturbance of glucose metabolism after obstructive jaundice may be the primary cause associated with the increase of expression levels of mRNA of G-6-P and PEPCK in hepatic tissues.