Study on the extraction of polyphenols from acorn seed using ultrasound-assisted two-phase aqueous system

Polyphenols from acorn seed was extracted by ultrasound-assisted two-phase aqueous system and through one-factor-at-a-time and orthogonal array design methods to optimize extracting condition.The results indicated that acetone concentration had significant influence on the yield of polyphenols,the influence of amount of(NH4)2SO4 and ultrasonic time and material-solvent ratio on the yield of polyphenols wasn't significant under the test range.The optimum conditions for the extraction of polyphenols were as follows,acetone concentration 80%,amount of(NH4)2SO4 0.16g/mL,ultrasonic time 25min,material-solvent ratio 1:45(W/V).Under the optimum conditions,through duplicated experiment the yield of polyphenols was 3.3033mg,RSD was 0.7674%,it indicated that the extraction rate of polyphenols was extremely stable.