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2004•Chinese journal of integrated traditional and Western medicineRequires access

Study on Mechanism of Norcantharidin Killing HeLa

Yanyu Wang

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Abstract

Objective To explore the mechanism of Norcantharidin(NCTD)in killing tumor cells.Methods Effect of NCTD on DNA replication was determined by flow cytometry;phase of NCTD effect determined by synchronized HeLa cells; changes of cytoplasmic microtubule construction and correlation of arrest and apoptosis in M stage shown by indirect im- muofluorescence.Results NCTD did not inhibit the proliferation of DNA and kill cells in G_1 and S stage.Changes of cy- toplasmic microtubule were obviously shown,arrest in M stage and increased deformity rate seen,HeLa cells were strong- ly killed at the concentration of 5 and 10μg/ml.NCTD of 2μg/ml did not kill the cells,but promoted the proliferation. Condusion NCTD had the bi-directional function of stimulating cell proliferation and killing tumor cells in a dose-de- pendant manner.Enough dosage of NCTD had anti-tumor effect in clinical application.

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What this paper is about

Objective To explore the mechanism of Norcantharidin(NCTD)in killing tumor cells.Methods Effect of NCTD on DNA replication was determined by flow cytometry;phase of NCTD effect determined by synchronized HeLa cells; changes of cytoplasmic microtubule construction and correlation of arrest and apoptosis in M stage shown by indirect im- muofluorescence.Results NCTD did not inhibit the proliferation of DNA and kill cells in G_1 and S stage.Changes of cy- toplasmic microtubule were obviously shown,arrest in M stage and increased deformity rate seen,HeLa cells were strong- ly killed at the concentration of 5 and 10μg/ml.NCTD of 2μg/ml did not kill the cells,but promoted the proliferation. Condusion NCTD had the bi-directional function of stimulating cell proliferation and killing tumor cells in a dose-de- pendant manner.Enough dosage of NCTD had anti-tumor effect in clinical application.

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Available abstract

Objective To explore the mechanism of Norcantharidin(NCTD)in killing tumor cells.Methods Effect of NCTD on DNA replication was determined by flow cytometry;phase of NCTD effect determined by synchronized HeLa cells; changes of cytoplasmic microtubule construction and correlation of arrest and apoptosis in M stage shown by indirect im- muofluorescence.Results NCTD did not inhibit the proliferation of DNA and kill cells in G_1 and S stage.Changes of cy- toplasmic microtubule were obviously shown,arrest in M stage and increased deformity rate seen,HeLa cells were strong- ly killed at the concentration of 5 and 10μg/ml.NCTD of 2μg/ml did not kill the cells,but promoted the proliferation. Condusion NCTD had the bi-directional function of stimulating cell proliferation and killing tumor cells in a dose-de- pendant manner.Enough dosage of NCTD had anti-tumor effect in clinical application.

Key concepts: HeLa, Apoptosis, Cell cycle, Flow cytometry, DNA synthesis, Chemistry, Microtubule, Cell growth

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