Effect of Eukaryotic Expression of survivin on Apoptosis of Ovarian Cancer Cell Induced by Trichostatin A

Objective To investigate the effect of survivin expression on apoptosis of ovarian cancer cells induced by Trichostatin A(TSA). Methods (1)A2780 cells were transfected with pcDNA3.1-survivin(full-length survivin cDNA) which has been successfully constructed in our laboratory. A2780 Cells transfected with pcDNA3.1 were cultured as controls.(2)The expression of survivin mRNA and protein was detected by RT-PCR and Western blot, respectively. (3)Two cell groups were treated with TSA and analyzed for suvival and apoptotic rate by MTT assay and flow cytometry (FACS). (4)Expression of survivin protein was detected under TSA treatment by Western blot. Results (1)The level of survivin mRNA and protein in transfected cells transfected with pcDNA3.1-survivin was significantly higher than that in control cells detected by RT-PCR and Western blot.(2)The viable rate of cells transfected with pcDNA3.1-survivin was obviously higher than that of control cells and the apoptotic rate was significantly lower than control detected by MTT assay and FACS, respectively(P0.05). The sensitivity of A2780 cells transfected with pcDNA3.1-survivin to TSA was greatly reduced.(3)The expression of survivin in A2780 cells obviously decreased with TSA incubation time. Conclusion Apoptosis of ovarian cancer cells induced by TSA may be related to the expression of survivin gene.