Genetic diversity and relationship analysis of oriental persimmon (Diospyros kaki) populations in Shandong by SSR

【Objective】 The objective of the experiment is to understand the diversity and genetic structure of oriental persimmon(Diospyros kaki) populations in Shandong.【Method】 The genetic diversity of 207 individuals from 8 persimmon(Diospyros kaki) populations in Shandong were investigated using SSR markers.The genetic parameters were analyzed using POPGENE 1.31 software,genetic variation of interpopulation and inner-population were analyzed by AREQUIN 2.0 version,and then the mean expected heterozygosity(He) and genetic differentiation coefficient were counted by TFPGA software.【Result】 As a result,128 bands,of which 105 were polymorphic loci,were amplified by 10 SSR primers selected from 51 markers.The relatively high levels of PPL(82%),Nei's gene diversity(Heranged from 0.153 8 to 0.247 6) and Shannon’s information diversity(H0ranged from 0.221 7 to 0.372 1) revealed relatively rich genetic diversity in the 8 persimmon populations.Analysis of molecular variance(AMOVA) showed that the variance component among populations contributes 24.17% at a significantly high level(P0.001).Furthermore,a low level of genetic variation among populations was detected based on the genetic distance(D 0.231 7),identity(I 0.783 2).Nei's genetic diversity analysis and AMOVA revealed that there was a higher level of genetic differentiation among populations(φST= 0.222 6,FST= 0.175 9).【Conclusion】The oriental persimmon populations in Shandong possess abundant genetic diversity,the genetic differentiation between populations may result from human selective pressures.