Diagnostic value of CellDetect~ staining technology in cervical exfoliative cytology

Purpose To observe the role of CellDetect staining technology in the diagnosis and differential diagnosis in exfoliative cell smears.Methods By CellDetect staining,the relevance of tinctorial and morphological data were analyzed between normal and neoplastic cells,and the diagnostic results were compared between CellDetect and HE stains in cervical intraepithelial neoplasia(CIN) and squmous cell carcinoma(SCC) from biopsied tissues.Results In conventional slides and TCT slides,CellDetect staining revealed cell morphological details,normal squamous cells all stained in blue or green,normal endocervical cells were red,and most dysplastic cells and cancer cells stained in pink or red.Most of the inflammatory cells had red or purple nuclei and blue or green cytoplasm,and erythrocytes stained green.The results of CellDetect staining technology in diagnosis of CIN and SCC were the same to that of HE staining.In tissue section from biopsy,CellDetect staining tinctorial characteristics in epithelium were considered to be correlated with the cells smears.However,CellDetect stain in evaluating SCC infiltration or stromal reaction was not satisfactory as compared with HE stain.Conclusion CellDetect staining provide a dual analysis of both color discrimination and morphological analysis,and it is suitable for different fixed cells.CellDetect staining would be an effective diagnostic method for early detection of cervical cancer and CIN.