The influence of endostatin on the expression of hypoxia-inducible factor-2 and vascular endothelial growth factor in ACHN RCC

Objective To study the inhibitory effect of endostatin gene therapy renal cell carcinoma (RCC) and the expression of HIF and VEGF.Methods ACHN-bearing nude mice were divided into three teams.Seven days later,ACHN-bearing nude mice were treated subcutaneously with 100 μl complex,included 30 μl SofastTM and 30 μg pSecES plasmids in experimental group,and 30 μl SofastTM and 30 μg pcDNA3.1 plasmids in control group,blank group was treated subcutaneously with 100 μl normal saline,and ACHN-bearing nude mice were treated with complex every 3-day,and were repeated for three times.After 34 days,the mice were sacrificed.Expression of HIF-2α and VEGF were detected by immunohistochemistry in three teams.Results The growth of ACHN RCC in the experimental group was significantly suppressed than the control and blank groups.There are HIF-2α and VEGF expression in the ACHN RCC of the experimental group less than the control and vacant groups.Conclusions Endostatin gene therapy can inhibit remarkably the growth of ACHN RCC in the nude mice model,and the expressions of HIF-2α and VEGF are decrease in ACHN RCC.The expression of VEGF is positively correlated with the expression of HIF-2α.