Preliminary analysis of mutation in pCR~(TM) II-FGFR1E recombinant plasmid

Objective: We found the pCR TM Ⅱ-FGFR1E recombinant plasmid changed in size from 4 700 bp to 9 000 bp, then we searched for the details Methods: Restriction enzyme digestion and Southern blot analysis were used. Results: The Sa1Ⅰ, XbaⅠ and BamHⅠ digestion fragments of mutated plasmid were different from those of the normal,but positive signals were found in the hybridization of mutated plasmid with FGFR1cDNA as probe. Conclusion: There are several restriction enzyme sites changing in the mutated plasmid, but FGFR1cDNA homologous sequences still remain.