EFFECTS OF INOSINE ON EXPRESSION OF Nogo-A mRNA AFTER CEREBRAL ISCHEMIC REPERFUSION IN RATS

ObjectiveTo investigate the expression of Nogo-A mRNA after cerebral ischemic reperfusion and the effects inosine on the expression. MethodsThe model of the middle cerebral artery occlusion (MCAO) and reperfusion was established in 60 adult female SD rats by nylon monofilament suture. The rats were randomly divided into treated and control groups. The rats in each group was further divided into 2-, 12-hour, 1-, 2-, 3-, 7-, and 14-day reperfusion subgroups, and the other four rats were sham-operated. Bederson and other neurological gradings were applied to evaluate neurological function, and in situ hybridization was used to examine the expression of Nogo-A mRNA in the brain tissues. ResultsNogo-A mRNA expression showed two peaks at 12 hours and two days in ischemic cortex and striatum in the control group. When given inosine,Nogo-A mRNA expression transiently increased at 12 hours but decreased at seven days in ischemic cortex, but increased at two and three days in ischemic striatum (t=1.93-14.96, P0.05). The neurological grade increased significantly at seven days(t=2.31,P0.05). Conclusion Inosine can improve neurological grade of MCAO rats. The enhanced neurological function might be partially caused by the down-regulation of Nogo-A mRNA. [