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Effect on Cytotoxicity by RNAi ERCC1 Gene Expression in Ovarian Cancer CelI Line

Jing Liu

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Abstract

Objective:To study the effect on drug resistance by RNAi mediated ERCC1 gene silencing in ovarian cancer cell line.Method:Small interference RNA targeted ERCC1 gene was synthesized in vitro and was transfected into COC1/DDP cell.The expression of ERCC1 was detected by semiquantitative reverse transcription-polymerase chain reaction(RT-PCR) and western blot.MTT test were applied to measure the inhibition combined with cisplatin of COC1/DDP strain.Result:After transfection of ERCC1-siRNA,mRNA and protein levels of ERCC1 gene in COC1/DDP cells were obviously reduced;ERCC1 gene combined with cisplatin increases the sensitivity to chemotherapy.Conclusion: ERCC1-siRNA could reduce the expression of ERCC1 gene and increase the sensitivity to cisplatin in COC1/DDP cell.

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What this paper is about

Objective:To study the effect on drug resistance by RNAi mediated ERCC1 gene silencing in ovarian cancer cell line.Method:Small interference RNA targeted ERCC1 gene was synthesized in vitro and was transfected into COC1/DDP cell.The expression of ERCC1 was detected by semiquantitative reverse transcription-polymerase chain reaction(RT-PCR) and western blot.MTT test were applied to measure the inhibition combined with cisplatin of COC1/DDP strain.Result:After transfection of ERCC1-siRNA,mRNA and protein levels of ERCC1 gene in COC1/DDP cells were obviously reduced;ERCC1 gene combined with cisplatin increases the sensitivity to chemotherapy.Conclusion: ERCC1-siRNA could reduce the expression of ERCC1 gene and increase the sensitivity to cisplatin in COC1/DDP cell.

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Available abstract

Objective:To study the effect on drug resistance by RNAi mediated ERCC1 gene silencing in ovarian cancer cell line.Method:Small interference RNA targeted ERCC1 gene was synthesized in vitro and was transfected into COC1/DDP cell.The expression of ERCC1 was detected by semiquantitative reverse transcription-polymerase chain reaction(RT-PCR) and western blot.MTT test were applied to measure the inhibition combined with cisplatin of COC1/DDP strain.Result:After transfection of ERCC1-siRNA,mRNA and protein levels of ERCC1 gene in COC1/DDP cells were obviously reduced;ERCC1 gene combined with cisplatin increases the sensitivity to chemotherapy.Conclusion: ERCC1-siRNA could reduce the expression of ERCC1 gene and increase the sensitivity to cisplatin in COC1/DDP cell.

Key concepts: ERCC1, Cisplatin, Transfection, Molecular biology, RNA interference, Gene silencing, Cytotoxicity, Medicine

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Effect on Cytotoxicity by RNAi ERCC1 Gene Expression in Ovarian Cancer CelI Line — Research Paper | ScholarLens