Immunity Evaluation on Inactivated Cellular Vaccine of H9N2 Subtype Avian Influenza Virus

Restricted by the economic and technological factors,avian influenza cellular vaccine has not been in market. Therefore,reassotant virus SH441HANAPR8 (NS mutant) that contained HA and NA genes from H9 subtype avian influenza virus acquired by cell culture was used in this paper. It was the best to mix SH441HANAPR8 (NS mutant) with formaldehyde to a final concentration of 0.1% and inactivated at 24 h. The inactivated SH441HANAPR8 (NS mutant) viruses were mixted with adjuvant Montanide ISA 70VG,and emulsified to inactivated vaccine. By chest muscles pathway,3-week-age specific pathogen free (SPF) chickens were immunized with H9 avian influenza virus inactivated vaccine (SH441HANAPR8 (NS mutant) in different doses of 0.05,0.1,0.2 and 0.3 mL per chicken. After 21 days post-immunization,these chickens were infected with avian influenza virus A/Chicken/Shanghai/441/2009 (H9N2). The results demonstrated that minimum immune dose of the vaccine was 0.2 mL per chicken. In addition,when HI titer was equal or greater than 25,challenge protection rate of vaccine was 100% for chicken.