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Optimizing on Liquid Culture Media of Cordyceps Sinensis Mycelia

Zheng Jun-ke

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Abstract

The liquid culture media of cordyceps sinensis mycelia was optimized by means of orthogonal analysis.The results showed that the optimal medium for the mycelia was 20% potato,0.08% beef extraxt 0.2% peptone,0.15 % KH2PO4,0.15 % MgSO4·7H2O,1.5% sucrose,2.5% glucose and pH natural.In the cultural condition of 23 ℃,130r/min and 4 d,the mycelia biomass could gain 19.5 g/ L,which was as 2 times higher than that before optimization.

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What this paper is about

The liquid culture media of cordyceps sinensis mycelia was optimized by means of orthogonal analysis.The results showed that the optimal medium for the mycelia was 20% potato,0.08% beef extraxt 0.2% peptone,0.15 % KH2PO4,0.15 % MgSO4·7H2O,1.5% sucrose,2.5% glucose and pH natural.In the cultural condition of 23 ℃,130r/min and 4 d,the mycelia biomass could gain 19.5 g/ L,which was as 2 times higher than that before optimization.

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Available abstract

The liquid culture media of cordyceps sinensis mycelia was optimized by means of orthogonal analysis.The results showed that the optimal medium for the mycelia was 20% potato,0.08% beef extraxt 0.2% peptone,0.15 % KH2PO4,0.15 % MgSO4·7H2O,1.5% sucrose,2.5% glucose and pH natural.In the cultural condition of 23 ℃,130r/min and 4 d,the mycelia biomass could gain 19.5 g/ L,which was as 2 times higher than that before optimization.

Key concepts: Mycelium, Cordyceps, Sucrose, Liquid culture, Food science, Biomass (ecology), Chemistry, Botany

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