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Isolation and Culture of Primordial Germ Cells from Chick Embryos

Li Bi

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Abstract

Blood (3 10 μL) of chick embryo was collected at 48,52,54,56 and 60 h for isolating PGCs.Concentrations of PGCs were 0 24%,1 21%,1 02%,0 64% and 0 27% respectively.PGCs concentrations increased from 0 01% of pre collection to 1 02% after collection.PGCs were cultured in the medium with TCM 199 containing 10% FCS,under 38 5℃ 5% CO 2 and saturation moisture in vitro .They could survive for about 3 4 days.

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What this paper is about

Blood (3 10 μL) of chick embryo was collected at 48,52,54,56 and 60 h for isolating PGCs.Concentrations of PGCs were 0 24%,1 21%,1 02%,0 64% and 0 27% respectively.PGCs concentrations increased from 0 01% of pre collection to 1 02% after collection.PGCs were cultured in the medium with TCM 199 containing 10% FCS,under 38 5℃ 5% CO 2 and saturation moisture in vitro .They could survive for about 3 4 days.

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Available abstract

Blood (3 10 μL) of chick embryo was collected at 48,52,54,56 and 60 h for isolating PGCs.Concentrations of PGCs were 0 24%,1 21%,1 02%,0 64% and 0 27% respectively.PGCs concentrations increased from 0 01% of pre collection to 1 02% after collection.PGCs were cultured in the medium with TCM 199 containing 10% FCS,under 38 5℃ 5% CO 2 and saturation moisture in vitro .They could survive for about 3 4 days.

Key concepts: Biology, Embryo, Germ, Andrology, In vitro, Embryo culture, Embryogenesis, Molecular biology

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