Anthocyanins inhibit functions of human platelet induced by adenosine diphosphate

Objective To explore the effect of anthocyanin cyanidin-3-glucoside(Cy-3g)on human platelet activation,aggregation and its mechanism.Methods The expression of activiated GPIIbIIIa and P-selectin on platelet surface were examined with flow cytometry in vitro.Platelet aggregation induced by adenosine diphosphate(ADP) was measured with platelet aggregometer;calcium level in the platelet cytoplasma was analyzed with Fluo-3 AM staining.Results The expression of activated GPIIbIIIa was significantly decreased by 50 μmol/L Cy-3g(73.92%±14.02%) compared with the control buffer(93.36%±2.02%,P0.05).The expression of P-selectin on platelet surface was not significantly different among the groups.Platelet aggregation of human platelet rich plasma(PRP)induced by ADP can be inhibited by Cy-3g at different doses(0.5 μmol/L,5 μmol/L,and 50 μmol/L),which were all significantly lower than that of the control(P0.001).The fluorescence intensity of calcium was decreased by 50 μmol/L Cy-3g(51.73±6.89%) in washed platelets(P0.01).Conclusion Cy-3g could inhibit the expression of platelet GPIIbIIIa and aggregation induced by ADP in vitro.The decreased platelet cytoplasmic calcium may be one of the mechanism.The results suggest that Cy-3g may have a positive role in the prevention of cardiovascular diseases.