Effects of Autophagy on Cisplatin Induced Cervical Cancer Hela Cells Death in Vitro

Objective:To investigate the effects of autophagy on cisplatin-induced cervical cancer Hela cell death,and observe impact of Beclin 1 on sensitivity of Hela cells to cisplatin in vitro. Methods:Hela cells were treated with cisplatin in different concentration, and MDC dyeing was explored to measure the formation of autophagic vacuoles and Western blot was used for detecting protein expression of Beclin 1 and LC3. The eukaryotic expression vector of Beclin 1 was transfected via lipofectamine into Hela cells. MTT assay was employed to observe the effect on the IC50value of cisplatin,and flow cytometry was employed to observe the percentage of autophagic cells and apoptotic cells. Results:The autophagy vesicles were increased and the expression of Beclin 1 and LC3 were significantly improved in a dose-dependent manner in Hela cells treated with cisplatin(P0.05). The eukaryotic expression vector pcDNA3.1(+)-Beclin1 significantly improved the expression of Beclin1 in Hela cells(P0.05). MTT assay showed the IC50value of cisplatin decreased from 1.0 mg/L to 0.5 mg/L(P0.05),and more autophagic cells and apoptotic cells were identified in pcDNA3.1(+)-Beclin1 group than the other groups. Conclusions:Autophagy play an important role in the process of cisplatin-induced Hela cells death,and Beclin 1 overexpression can increase the sensitivity of Hela cells to cisplatin.