Research on total flavonoids extraction procedure from Huai chrysanthemum by water bath

Using orthogonal design test to optimize total flavonoids extraction procedure from Huai chrysanthemum by water bath with rutin as reference substance,and determine the content of total flavonoids at 505 nm by using UV spectrophotometry.The results showed that total flavonoids in the range of 0~50 g/mL,there was a good linear relationship between absorbency and concentration.The optimum extraction conditions of total flavonoids extraction procedure from Huai chrysanthemum by water bath was A1B1C3,temperature was 60℃,ethanol concentration was 60%,the ratio of material and solvent was 1∶10,extraction 2 times for 1 hour,extraction rate was 109.74 mg/g.The regression equation was y=0.01058x-0.01233,R2=0.9973,average recovery was 98.16%,and RSD was 0.210%.This method is low cost,no pollution,easy to operation,and results accurately.This method can be used as the quality control method of the Huai chrysanthemum.