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Isozyme analysis of Triplophysa xiangxiensis population.

Zheng Zhang

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Abstract

By using vertical polyacrylamide gel electrophoresis(PAGE) ,the genetic diversity of Triplophysa xiangxiensis(cave fish) population was studied. A total of 26 loci were detected in 7 isozymes(LDH,MDH,ME,EST,ADH,GDH,and G-6-PDH) of six tissues(muscle,heart,liver,brain,spleen,and kidney) ,among which,Ldh-3,Ldh-4,s-Mdh and m-Mdh were polymorphic,with the mean proportion of polymorphic loci being 15.38%. The observed heterozygosity was 0.0398,expected heterozygosity was 0.0411,and genetic deviation index(D value) was-0.0316,suggesting that T. xiangxiensis population had a lower genetic diversity and a higher genetic purity.

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What this paper is about

By using vertical polyacrylamide gel electrophoresis(PAGE) ,the genetic diversity of Triplophysa xiangxiensis(cave fish) population was studied. A total of 26 loci were detected in 7 isozymes(LDH,MDH,ME,EST,ADH,GDH,and G-6-PDH) of six tissues(muscle,heart,liver,brain,spleen,and kidney) ,among which,Ldh-3,Ldh-4,s-Mdh and m-Mdh were polymorphic,with the mean proportion of polymorphic loci being 15.38%. The observed heterozygosity was 0.0398,expected heterozygosity was 0.0411,and genetic deviation index(D value) was-0.0316,suggesting that T. xiangxiensis population had a lower genetic diversity and a higher genetic purity.

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Available abstract

By using vertical polyacrylamide gel electrophoresis(PAGE) ,the genetic diversity of Triplophysa xiangxiensis(cave fish) population was studied. A total of 26 loci were detected in 7 isozymes(LDH,MDH,ME,EST,ADH,GDH,and G-6-PDH) of six tissues(muscle,heart,liver,brain,spleen,and kidney) ,among which,Ldh-3,Ldh-4,s-Mdh and m-Mdh were polymorphic,with the mean proportion of polymorphic loci being 15.38%. The observed heterozygosity was 0.0398,expected heterozygosity was 0.0411,and genetic deviation index(D value) was-0.0316,suggesting that T. xiangxiensis population had a lower genetic diversity and a higher genetic purity.

Key concepts: Loss of heterozygosity, Isozyme, Genetic diversity, Biology, Population, Polyacrylamide gel electrophoresis, Genetic variability, Genetic distance

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Isozyme analysis of Triplophysa xiangxiensis population. — Research Paper | ScholarLens