ScholarLens Back to search
2013•Journal of Anhui Polytechnic UniversityRequires access

Expression and purification of thymosin α1 in Pichia pastoris

Bin Tang

Open publisher page 0 citations

Abstract

Thymosin α1(Tα1) was prepared by genetic engineering strain fermentation in this study.The artificial synthesized Tα1 gene was inserted into the pPIC9K plasmid to construct expression vector pPIC9K-Tα1.It was transformed to Pichia pastoris GS115,the recombinant strain was induced by methanol.The supernatant of its fermentation medium was purified by DEAE52 and SephadexG-25.Both Tricine-SDS-PAGE and HPLC indicated that the Tα1 protein was expressed successfully.The expression level was about 4.8 mg/L.

About this research paper

What this paper is about

Thymosin α1(Tα1) was prepared by genetic engineering strain fermentation in this study.The artificial synthesized Tα1 gene was inserted into the pPIC9K plasmid to construct expression vector pPIC9K-Tα1.It was transformed to Pichia pastoris GS115,the recombinant strain was induced by methanol.The supernatant of its fermentation medium was purified by DEAE52 and SephadexG-25.Both Tricine-SDS-PAGE and HPLC indicated that the Tα1 protein was expressed successfully.The expression level was about 4.8 mg/L.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Thymosin α1(Tα1) was prepared by genetic engineering strain fermentation in this study.The artificial synthesized Tα1 gene was inserted into the pPIC9K plasmid to construct expression vector pPIC9K-Tα1.It was transformed to Pichia pastoris GS115,the recombinant strain was induced by methanol.The supernatant of its fermentation medium was purified by DEAE52 and SephadexG-25.Both Tricine-SDS-PAGE and HPLC indicated that the Tα1 protein was expressed successfully.The expression level was about 4.8 mg/L.

Key concepts: Pichia pastoris, Recombinant DNA, Expression vector, Fermentation, Pichia, Plasmid, Chemistry, Molecular biology

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Expression and purification of thymosin α1 in Pichia pastoris — Research Paper | ScholarLens