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Detection of Strawberry mottle virus by NASBA technology
Hongyi Yang
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Abstract
Nucleic acid sequence based amplification (NASBA) is a single-step isothermal RNA-specific amplification process that amplifies mRNA in a dsDNA background. In this study,NASBA was applied to detect Strawberry mottle virus (SMoV) in strawberry plants. After the sequences of 14 SMoV isolates were analyzed,the primers for detection of SMoV by NASBA were designed and screened. The suitable temperature of NASBA reaction for detecting SMoV was 40 ℃. Using 9 strawberry samples as materials,the result of detection of SMoV by NASBA was identical with the detection result by RT-PCR. It means that the primary system of detection of SMoV by NASBA was developed in this study. It is the first report of SMoV detection by NASBA.