Optimization of Microwave-assisted Extraction of Flavones from Celery Using Response Surface Analysis

The extraction process of total flavones from celery by microwave-assisted extraction using response surface analysis(RSA) was studied.Based on single factor test,Box-Benhnken Design(BBD) was applied to the study of the effects of microwave power,extraction time and liquid-solid ratio on the extraction rate of total flavones.Quadratic regression model equation between the extraction rate of total flavones and the factors was established,and the regression model was significant.The optimal extraction conditions were then determined by RSA as follows: 80% ethanol as solvent,microwave power 520 W,extraction time 9 min and liquid-solid ratio 32∶1 ml/g.In this case,the extraction rate of total flavones from celery sample reaches 2.443 mg/g.