ScholarLens Back to search
2011•Zhongguo kangfu lilun yu shijianRequires access

Protecting Effect of Porphyra Yezoensis Polysaccharide on Cerebrum Tissue of Diabetic Rats

Lin Fen

Open publisher page 2 citations

Abstract

Objective To observe the effect of porphyra yezoensis polysaccharide(PYP) on cerebrum tissue of diabetic rats and apopto-sis.Methods 60 Wistar rats were randomly divided into normal control group,diabetic model group,and treated groups respectively with PYP(0.25 g/kg × d),PYP(0.5 g/kg × d),and PYP(1 g/kg × d).The cerebrum pathologic changes were observed by HE staining method,and the number of apoptotic cells was observed by electrophoresis and the mRNA expression of p53 and bcl-2 was confirmed by RT-PCR.Re-sults PYP improved the cerebrum pathologic changes of diabetic rats.With comparison to the diabetic model group,the cerebrum patholog-ic change in the treated groups also improved.And the number of apoptotic cells,the expression of p53 in cerebrum tissue decreased and ex-pressions of bcl-2 rose.Conclusion PYP can decrease the expression of p53 in cerebrum tissue and rise expressions of bcl-2 so as to protect brain tissues from cerebral ischemia.

About this research paper

What this paper is about

Objective To observe the effect of porphyra yezoensis polysaccharide(PYP) on cerebrum tissue of diabetic rats and apopto-sis.Methods 60 Wistar rats were randomly divided into normal control group,diabetic model group,and treated groups respectively with PYP(0.25 g/kg × d),PYP(0.5 g/kg × d),and PYP(1 g/kg × d).The cerebrum pathologic changes were observed by HE staining method,and the number of apoptotic cells was observed by electrophoresis and the mRNA expression of p53 and bcl-2 was confirmed by RT-PCR.Re-sults PYP improved the cerebrum pathologic changes of diabetic rats.With comparison to the diabetic model group,the cerebrum patholog-ic change in the treated groups also improved.And the number of apoptotic cells,the expression of p53 in cerebrum tissue decreased and ex-pressions of bcl-2 rose.Conclusion PYP can decrease the expression of p53 in cerebrum tissue and rise expressions of bcl-2 so as to protect brain tissues from cerebral ischemia.

Why it matters

OpenAlex reports 2 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To observe the effect of porphyra yezoensis polysaccharide(PYP) on cerebrum tissue of diabetic rats and apopto-sis.Methods 60 Wistar rats were randomly divided into normal control group,diabetic model group,and treated groups respectively with PYP(0.25 g/kg × d),PYP(0.5 g/kg × d),and PYP(1 g/kg × d).The cerebrum pathologic changes were observed by HE staining method,and the number of apoptotic cells was observed by electrophoresis and the mRNA expression of p53 and bcl-2 was confirmed by RT-PCR.Re-sults PYP improved the cerebrum pathologic changes of diabetic rats.With comparison to the diabetic model group,the cerebrum patholog-ic change in the treated groups also improved.And the number of apoptotic cells,the expression of p53 in cerebrum tissue decreased and ex-pressions of bcl-2 rose.Conclusion PYP can decrease the expression of p53 in cerebrum tissue and rise expressions of bcl-2 so as to protect brain tissues from cerebral ischemia.

Key concepts: Cerebrum, Apoptosis, Staining, Brain tissue, Polysaccharide, Messenger RNA, Necrosis, Biology

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Protecting Effect of Porphyra Yezoensis Polysaccharide on Cerebrum Tissue of Diabetic Rats — Research Paper | ScholarLens