Preparation and Preliminary Identification of Monoclonal Antibodies Against Human Endothelial Cell Specific Molecule-1

The human endothelial cell specific molecule-1 (ESM-1) was used as antigen to prepare monoclonal antibodies by means of the B lymphocyte hybridoma technique. Seven strains of hybridoma cells secreting anti human ESM-1 stably were obtained after PEG fusion and screening by limited dilution,in which the McAb3A7 secreted by one of the hybridoma cells showed the highest specificity with the agglutination titer of 1∶6 000. The subtype of the secreted antibody was IgG2b. This monoclonal antibody could recognize specifically the ESM-1 both in endothelial cells and supernatants of cell cultures. The distribution of ESM-1 was mainly in the cytoplasm of endothelial cells,and located in the vasculoendothelial cells of renal tissues. Higher expression of ESM-1 in renal cell carcinoma than that of normal renal tissues was also noticed. These results indicate that this monoclonal antibody shows both high sensitivity and specificity.