Optimization of exogenous gene transfection in human umbilical vein endothelial cells

Objective To compare the efficiency of cationic liposome- and electroporalion-mediated exogenous gene transfection in human umbilical vein endothelial cells(HUVEC ),and optimize the transfection condition.Methods HUVEC cells were transfected with plasmid DNA,earned green fluorescent protein(GFP) gene,by Lipofectarnin 2000 or electroporation.The GFP expression and transfection efficiency were examined by fluorescent microscope at 48 h after transfection.Results The hypofluorescence signal was observed in the Lipofectamine~(TM) 2000-treated group,however the hyperfluorescence signal was detected in the electroporation group.The optimal condition in the electroporation group included the electric field strength 1 100 V,pulse time 20 ms and electric shock 2 times.Conclusion The efficiency of exogenous gene transfection with electroporation is higher than cationic liposome,which is suitable for exogenous gene transfection in HUVEC cells.