In silico cloning and bioinformatic analysis of DYRK2 gene in cattle

Using human DYRK2 cDNA sequence as a probe, screening homology sequences in the EST database, the DYRK2 gene from cattle was cloned by silico cloning. Sequence analysis showed that cattle DYRK2 cDNA contained an open reading frame(ORF) with 1 581 bp which encoded 526 amino acids. Protein analysis found that the molecular formula of encoded protein DYRK2 of cattle was C2632H4204N762O761S26, with weight of 59 532.5 and theoretical pI of 9.53. The result indicated that the nucleotide sequences of the coding region between cattle, sheep, people and other animals were quite conservative.