Expression of hepatocyte growth factor receptor in flbroblasts induced by high glucose

Objective To examine the expression of hepatocyte growth factor(HGF) receptor (c-met) and investigate the changes in activity of the HGF/c-met system in human kidney fibroblast by high glucose. Methods HGF and c-met mRNA levels in fibroblast induced by high glucose were detected by RT-PCR. C-met protein was examined by Western blotting. At the same time, the expression of TGF-β and c-met in the exogenous HGF and treating with anti-c-met antibody in vitro were measured. Results Extremely rapid induction of HGF and c-met mRNA was observed at the first six hours by high glucose. On the other hand, both c-met mRNA and c-met protein were markedly increased. HGF (50 ng/ml) induced the expression of c-met ( P 0. 05) and also decreased the level of TGF-β ( P 0. 05). When the action of c-met was blocked by anti-c-met antibody, HGF didn't show the effect before. Conclusion Local up-regulation of c-met is crucial to renal repair, which may be associated with the increaseing activity of HGF/c-met system.