A Study on the Effectiveness of the Chimeric HA1 DNA Vaccine of Influenza A and B Virus

The HA1 fragments from influenza A and B virus HA genes were cloned together into an expression vector to construct a chimeric DNA vaccine.Mice were immunized twice intramuscularly by electroporation with the constructed plasmid.One week after the booster,the mice were challenged with lethal dose of homologous type A or B virus.The effectiveness of the chimeric DNA was evaluated by the antibody response and the protective abilities,including the survival rate,lung virus titer and body weight change.The results showed that the immunized mice could be protected against not only homologous A virus but also B virus,indicating the ability of the chimeric DNA to provide cross protection.