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Construction of recombinant adenovirus with IBDV VP2 gene

Chunhong Zhang

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Abstract

The recombinant plasmid pAdlox VP2 was obtained after the VP2 gene of infectious bursal disease virus (IBDV) cloned by RT-PCR was sub-cloned into the transfer vector pAdlox. The pAdlox VP2 was transfered into Cre8 cells together with the wild-type adenovirus ψ5 DNA to express the VP2 gene of IBDV. The result demonstrated that the recombinant adenovirus containing the IBDV VP2 gene was (constructed) successfully, and a 37 ku protein was expressed successfuly in the Cre8 cells, which was (confirmed) by Western-blotting method.

About this research paper

What this paper is about

The recombinant plasmid pAdlox VP2 was obtained after the VP2 gene of infectious bursal disease virus (IBDV) cloned by RT-PCR was sub-cloned into the transfer vector pAdlox. The pAdlox VP2 was transfered into Cre8 cells together with the wild-type adenovirus ψ5 DNA to express the VP2 gene of IBDV. The result demonstrated that the recombinant adenovirus containing the IBDV VP2 gene was (constructed) successfully, and a 37 ku protein was expressed successfuly in the Cre8 cells, which was (confirmed) by Western-blotting method.

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Available abstract

The recombinant plasmid pAdlox VP2 was obtained after the VP2 gene of infectious bursal disease virus (IBDV) cloned by RT-PCR was sub-cloned into the transfer vector pAdlox. The pAdlox VP2 was transfered into Cre8 cells together with the wild-type adenovirus ψ5 DNA to express the VP2 gene of IBDV. The result demonstrated that the recombinant adenovirus containing the IBDV VP2 gene was (constructed) successfully, and a 37 ku protein was expressed successfuly in the Cre8 cells, which was (confirmed) by Western-blotting method.

Key concepts: Infectious bursal disease, Recombinant DNA, Virology, Biology, Molecular biology, Plasmid, Gene, Virus

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