Effects of adipose derived stem cells transplantation on the expression of TNF-α and MBP in sciatic nerve of rats with diabetic peripheral neuropathy

Objective To investigate the effects of transplantation of adipose-derived stem cells(ADSC) on the expression of tumor necrosis factor-α(TNF-α) and myelin basic protein(MBP) in the sciatic nerve of rats with diabetic peripheral neuropathy(DPN). Methods The rats were randomly divided into normal group, model group, ADSC group. The rat model of diabetic peripheral neuropathy was established by injection of streptozotocin peritoneally. ADSC suspension was infused into the caudal vein in ADSC group. 14 days after modeling, the blood sugar level and conduction velocity of sciatic nerve were measured, the mRNA expression of TNF-α and MBP in the sciatic nerve was detected by real time-PCR.Results Compared with normal group, the blood sugar level was increased and the conduction velocity of sciatic nerve was decreased in model group and ADSC group( 0.05), the expression levels of TNF-α mRNA and MBP mRNA in sciatic nerve were increased( 0.05). The conduction velocity was higher( 0.05), and the expression levels of TNF-α mRNA and MBP mRNA in sciatic nerve were decreased( 0.05) in ADSC group than in the model group. Conclusion ADSC could downregulate the expression levels of TNF-α mRNA and MBP mRNA in sciatic nerve of rats with diabetic peripheral neuropathy.