Effects of 2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-Glucoside on Apoptosis of HUVEC Induced by H_2O_2 and the Mechanism Involved NF-κB Signaling Pathway

Aim To investigate the protective effect of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside(TSG) on human umbilical vein endothelial cells(HUVEC) apoptosis induced by H_2O_2 and further explore whether this effect is related to the NF-κB signaling pathway and the expression of apoptosis-related protein. Methods HUVEC were divided into three groups: control group,H_2O_2 group and TSG group.The proliferation and apoptosis rates of HUVEC were detected by MTT and flow cytometry respectively.The protein expression of NF-κB p65,IκB and bcl-2 were assessed by Western blot. Results According to the MTT and flow cytometry,the proliferation rate of HUVEC decreased significantly compared with control group(P0.01),while the apoptosis rate increased obviously(P0.01) after treating with H_2O_2.The proliferation rate of TSG pretreatment group increased significantly(P0.01) while the apoptosis rate decreased obviously(P0.01) compared with H_2O_2 group.Compared with H_2O_2 group,Western blot analysis revealed that the expression of NF-κB p65 was declined and the level of IκB and bcl-2 were increased significantly after pretreated by TSG(P0.01). Conclusions TSG has a protective effect on the HUVEC apoptosis induced by H_2O_2 and this molecular mechanism may be associated with its inhibiting the activation of NF-κB/IκB signaling pathway and the up-regulating expression of bcl-2 protein.