Comparison of different kinds of syphilis detection in clinical application

Objective To compare four kinds of syphilis testing methods of antibody of treponema pallidum particle agglutination(TPPA),treponema pallidum enzyme-linked immunosorbent assay(TP-ELISA),rapid plasma reagin circle card test(RPR),colloidal gold rapid testing(SYP),and choose a model for clinical syphilis testing. Methods(1) Four methods were used to detect more than 135 cases are known to out-of Binzhou Medical College Hospital from October 2012 to May 2013 among patients diagnosed with syphilis. Among those patients,in which 46 cases of primary syphilis,39 cases of secondary syphilis,tertiary syphilis in 5 cases,45 cases of latent syphilis.(2) From 2012 October to May 2013 patients in our hospital before surgery or blood transfusion before the blood samples were drawn 32066copies,first with the TP-ELISA assay and found positive for 266 cases,and then TPPA,RPR,SYP were detected.Results(1) The first set of test results: TPPA,ELISA,RPR,SYP four species sensitivity of syphilis testing methods were 98.78%,98.78%,78.52%,91.11%. TPPA,TP-ELISA had no significant difference,TPPA,ELISA,RPR,SYP4 kinds of syphilis testing methods between the first two and last two and last two has significant differences between each other.(2)The second group of test results: 266 cases were TP-ELISA positive samples with TPPA,RPR,SYP assay,the positive rate was 85%,69%,79%. TPPA,RPR,SYP three tests were significantly different between each other. Conclusion TPPA method applies to be the confirmatory test after other methods,or a smaller workload of medical institutions. RPR for primary syphilis screening and therapeutic evaluation of primary hospital patients. TPELISA method is suitable for the larger medical institutions work screening test. SYP method is suitable for primary care hospital or emergency testing,but the test should be re-examination by the application of other methods.