miR-126 expression and its effect on human colon cancer cells in the biological behavior of colon cancer cells

Objective To investigate the expression of MicroRNA-126(miR-126) in human colon cancer cells and its effect on biological behavior of colon cancer cells.Methods Sixty patients with colon cancer were enrolled in the study.Fluorescence PCR was used to detect the expression of miR-126 in the cancerous tissues and in the tissues adjacent to carcinoma.The expression of miR-126 in the cancerous tissues paired up with the expression of miR-126 in the tissues adjacent to carcinoma,and there were 60 pairs in total.The expression of miR-126 in 5 colon cancer cell lines(SW480,HT29,HCT116,LOVO,SW620) was also detected.The proliferation,migration and invasion ability of the cells were measured using CCK 8 assay,scratch experiments and Transwell assay,respectively.The effect of miR-126 on the biological behaviors of human colon cancer cells was further studied through the experiments in vitro.Results Compared with adjacent normal tissue cells,miR-126 was decreased(95.0%vs.55.0%,P0.05) in colon cancer cells,and were obviously when cancer metastasis or invasion is particularly evident,when compared with those without metasis,there were significant difference(25.0%vs.75.0%,P0.05).Studies have shown that miR-126 can inhibit the growth of SW480 cells and their invasion and metastasis,Transwell assay with matrigel showed the migration cell number in transfection group and blank control group was(10.0±4.3)and(258.0±30.6),while Transwell assay without matrigel showed the migration cell number was(84.0±13.2) and(335.0±27.3)respectively,there were significant differences(P0.05).miR-126 expression can enhance the sensitivity of cancer cells to oxaliplatin.Conclusion miR-126 can inhibit the development of colon cancer cells,the impact of a variety of strains of the biological behavior of colon cancer cells.