Effects of Anisodamine on Human Umbilical Vein Endothelial Cells′ Apoptosis Induced by Hypoxia-reoxygenation

Objective To explore the influence of anisodamine on human umbilical vein endothelial cells′(HUVECs) apoptosis induced by hypoxia and reoxygenation.Methods The HUVECs were divided into groups control(group A),hypoxia-reoxygenation 0.9% saline(group B),hypoxia-reoxygenation epinephrine(group C),hypoxia-reoxygenation Ani(group D),hypoxia-reoxygenation epinephrine plus Ani(group E).The cell apoptoses were detected by fluorescence flow cytometry,the gene expression of Bcl-2 by reverse transcription-polymerase chain reaction(RT-PCR).Results There was significant difference in apoptotic indexes(AI) of HUVECs at hours 12,24 of reoxygenation between 5 groups(P0.05),group B were significantly different from groups C,D,E(P0.05),group C not from group D(P0.05),but groups C,D from group E(P0.05).There was significant difference in Bcl-2mRNA level between 5 groups at hours 12,24 of reoxygenation(P0.05),and Bcl-2mRNA expression increased more significantly in group Ethan in other groups(P0.05).Conclusion Ani can reduce apoptosis of HUNVECs after hypoxia-reoxygenation.Ani protects the function of endothelia cells after hypoxia-reoxygenation by influencing the mRNA expression of Bcl-2.