Flow Cytometry Detection of the Membrane Antigens and Cytoplasmic Antigens in Acute Leukemia

Objective To detect the cell membrane and cell plasma antigens by flow cytometry and(evaluate) the role of these antigens in acute leukemia(AL) immunophenotyping.Methods The bone marrow or blood in 290 cases of patients with AL were tested by three color direct immunofluorescence staining to determine the immunophenotype by flow cytometry. Results The positive expression rate of CD_(13) and CD_(33) found in acute myelogenous leukemia(AML)-M_1,AML-M_2,AML-M_3,AML-M_5 was 89.6% and 77.6% and myeloperoxidase(MPO) expressions were 99.25% in 134 cases with AML.In 125 B-ALL patients, the positive rate in CyCD_(79a) was 97.6%,CD_(19) 84%,CD_(22) 84%,CD_(10) 68.8%.In 31 T-ALL patients,the positive rate in CyCD_3 was 96.8%;CD_2 71%;CD_3 80.6%;CD_5 87.1%;CD_7 83.9%.The positive expression rate of CD_(34) was 47% and 62.8% in 134 cases with AML and 156 cases with ALL.Conclusion Detection of membrane antigens and cytoplasmic antigens by flow cytometry will benefit the classification of acute leukemia.