2012•Chongqing Yike Daxue xuebaoRequires access

Construction of subtracted cDNA library of diferentially expressed genes of multidrug resistant tubercle bacillus by suppression subtracted hybridization

Zhu Chao-min

Abstract

Objective:To build the subtracted cDNA library of differentially expressed genes of multidrug-resistant tubercle bacillus(MDR-TB)and to further discuss the molecular mechanism of MDR-TB.Methods:Tester was MDR-TB and Driver was sensitive tuberculosis.Suppression subtractive hybridization(SSH) and T/A cloning technology were done to build the subtracted cDNA library of differentially expressed genes of MDR-TB.Results:The subtracted cDNA library of differentially expressed genes of MDR-TB was successfully built and 113 differentially expressed cDNA fragements of MDR-TB were obtained.Sequencing and homology analysis showed that 5 of them were novel cDNA sequences and 5 sequenced genes were reported to be related with MDR in TB.Conclusions:SSH is an effective method for screening new function genes.Many genes both known and unknown are in correlation with MDR in TB.Discovery of these genes provides a solid foundation for the explanation of MDR mechanism in TB.

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Key concepts: Suppression subtractive hybridization, Complementary DNA, Gene, cDNA library, Biology, Multiple drug resistance, Genetics, Homology (biology)