ScholarLens Back to search
2003•Huaxi yaoxue zazhiRequires access

Extracellular cisplatin inhibit K_(Ca) current from isolated guinea pig cochlear outer hair cells

Ding-qiang Huang

Open publisher page 0 citations

Abstract

OBJECTIVE The effects of cisplatin on K Ca currents were investigated in dissociated cochlear outer hair cells of the guinea pig. METHODS Guinea pig was decapitated and the temporal bone was quickly removed. Then the lateral wall of the cochlea was removed. The organ of Corti were dissected out under a dissection microscope. Single cells were obtained by digestion with trypsin and gentle mechanical trituration. Patch clamp technique were used to record K Ca current from isolated guinea pig cochlear outer hair cells and determine the effect of cisplatin on it.RESULTS The results showed that the activation of K Ca channels were inhibited by extracellular cisplatin in concentration of 10 -5 mol·L -1 .CONCLUSION This finding demonstrates that extracellular cisplatin depress K Ca current in the guinea pig cochlear outer hair cells.

About this research paper

What this paper is about

OBJECTIVE The effects of cisplatin on K Ca currents were investigated in dissociated cochlear outer hair cells of the guinea pig. METHODS Guinea pig was decapitated and the temporal bone was quickly removed. Then the lateral wall of the cochlea was removed. The organ of Corti were dissected out under a dissection microscope. Single cells were obtained by digestion with trypsin and gentle mechanical trituration. Patch clamp technique were used to record K Ca current from isolated guinea pig cochlear outer hair cells and determine the effect of cisplatin on it.RESULTS The results showed that the activation of K Ca channels were inhibited by extracellular cisplatin in concentration of 10 -5 mol·L -1 .CONCLUSION This finding demonstrates that extracellular cisplatin depress K Ca current in the guinea pig cochlear outer hair cells.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

OBJECTIVE The effects of cisplatin on K Ca currents were investigated in dissociated cochlear outer hair cells of the guinea pig. METHODS Guinea pig was decapitated and the temporal bone was quickly removed. Then the lateral wall of the cochlea was removed. The organ of Corti were dissected out under a dissection microscope. Single cells were obtained by digestion with trypsin and gentle mechanical trituration. Patch clamp technique were used to record K Ca current from isolated guinea pig cochlear outer hair cells and determine the effect of cisplatin on it.RESULTS The results showed that the activation of K Ca channels were inhibited by extracellular cisplatin in concentration of 10 -5 mol·L -1 .CONCLUSION This finding demonstrates that extracellular cisplatin depress K Ca current in the guinea pig cochlear outer hair cells.

Key concepts: Guinea pig, Chemistry, Organ of Corti, Extracellular, Cochlea, Outer hair cells, Cisplatin, Inner ear

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Extracellular cisplatin inhibit K_(Ca) current from isolated guinea pig cochlear outer hair cells — Research Paper | ScholarLens