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Molecular Cloning of Phenylalanine Ammonia-lyase in Ginkgo biloba

Shuiyuan Cheng

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Abstract

According to the conservative domain of some other plants DNA sequences of PAL gene,a pair of degenerate primer was designed.A special DNA fragment was obtained by PCR amplifying,which length is 862 bp,and it is named Gbpal1.We analysed the nucleotide and amino acid sequence of smilarity to the corresponding gene of other organism,the results showed that Gbpal1 is a partial PAL gene of Ginkgo biloba.The sequence had been embodied in Genebank,and Accession NO.is AY578145.The southern blot analysis indicated that Ginkgo biloba PAL belonged to a multigene family.

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What this paper is about

According to the conservative domain of some other plants DNA sequences of PAL gene,a pair of degenerate primer was designed.A special DNA fragment was obtained by PCR amplifying,which length is 862 bp,and it is named Gbpal1.We analysed the nucleotide and amino acid sequence of smilarity to the corresponding gene of other organism,the results showed that Gbpal1 is a partial PAL gene of Ginkgo biloba.The sequence had been embodied in Genebank,and Accession NO.is AY578145.The southern blot analysis indicated that Ginkgo biloba PAL belonged to a multigene family.

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Available abstract

According to the conservative domain of some other plants DNA sequences of PAL gene,a pair of degenerate primer was designed.A special DNA fragment was obtained by PCR amplifying,which length is 862 bp,and it is named Gbpal1.We analysed the nucleotide and amino acid sequence of smilarity to the corresponding gene of other organism,the results showed that Gbpal1 is a partial PAL gene of Ginkgo biloba.The sequence had been embodied in Genebank,and Accession NO.is AY578145.The southern blot analysis indicated that Ginkgo biloba PAL belonged to a multigene family.

Key concepts: Ginkgo biloba, Ginkgo, Biology, Gene, Molecular cloning, Primer (cosmetics), Genetics, Nucleic acid sequence

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