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Regulation of PTA1 promoter by AP1

Jian‐Ping Jin

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Abstract

AIM: To identify the transcription start site of human PTA1 gene and to investigate the regulation of PTA1 promoters by AP1. METHODS: The transcription start site was cloned by 5′-RACE method. The AP1 expression vector and the reporter vector containing PTA1 promoter were cotransfected into HHCC cell line. The luciferase activity of transfected HHCC was determined after 48 h culture. RESULTS: The transcription start site was located at the 229 bp upstream of ATG. AP1 up-regulated PTA1 promoter P1 and P2, while the transcription factor ets1 had different regulatory effects on PTA1 promoter P1 and P2 when cotransfected with AP1. CONCLUSION: The transcription start site of PTA1 is at the 229 bp upstream of ATG and the PTA1 promoters P1 and P2 can be regulated by AP1 and ets1.

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What this paper is about

AIM: To identify the transcription start site of human PTA1 gene and to investigate the regulation of PTA1 promoters by AP1. METHODS: The transcription start site was cloned by 5′-RACE method. The AP1 expression vector and the reporter vector containing PTA1 promoter were cotransfected into HHCC cell line. The luciferase activity of transfected HHCC was determined after 48 h culture. RESULTS: The transcription start site was located at the 229 bp upstream of ATG. AP1 up-regulated PTA1 promoter P1 and P2, while the transcription factor ets1 had different regulatory effects on PTA1 promoter P1 and P2 when cotransfected with AP1. CONCLUSION: The transcription start site of PTA1 is at the 229 bp upstream of ATG and the PTA1 promoters P1 and P2 can be regulated by AP1 and ets1.

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Available abstract

AIM: To identify the transcription start site of human PTA1 gene and to investigate the regulation of PTA1 promoters by AP1. METHODS: The transcription start site was cloned by 5′-RACE method. The AP1 expression vector and the reporter vector containing PTA1 promoter were cotransfected into HHCC cell line. The luciferase activity of transfected HHCC was determined after 48 h culture. RESULTS: The transcription start site was located at the 229 bp upstream of ATG. AP1 up-regulated PTA1 promoter P1 and P2, while the transcription factor ets1 had different regulatory effects on PTA1 promoter P1 and P2 when cotransfected with AP1. CONCLUSION: The transcription start site of PTA1 is at the 229 bp upstream of ATG and the PTA1 promoters P1 and P2 can be regulated by AP1 and ets1.

Key concepts: AP-1 transcription factor, Promoter, Transcription factor, Transcription (linguistics), Molecular biology, Reporter gene, Gene, Luciferase

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