Studies on genetic polymorphism at four microsatellite loci in Bore goats
Binyun Cao
Abstract
Binyun Cao
Abstract
According to comparative genomics,the four microsatellite loci including OarAE101,OarHH55,BM1329 and BMS2508 that were linked to the fecundity gene FecB in the sheep chromosome 6 were selected to obtain molecular markers in goat.The frequency of allele,polymorphism information content(PIC),number of effective alleles(Ne) and heterozygosity(He) of corresponding microsatellite locis in Boer goat were detected.The results indicated that there were genetic polymorphisms at these four microsatellite loci.PIC,Ne,and He at OarAE101 was 0.847 4,6.553 1,0.862 8,respectively;PIC,Ne,and He at OarHH55 was 0.767 6,4.302 9,0.796 8,respectively;PIC,Ne,He at BM1329 was 0.841 4,6.305 2,0.857 6,respectively;the PIC,Ne,He at BMS2508 was 0.783 1,4.610 4,0.809 6,respectively in Boer goats.The four microsatellite loci were height polymorphic loci which could be used for genetic polymorphism evaluation and study of the fecundity trait in Boer goats.
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According to comparative genomics,the four microsatellite loci including OarAE101,OarHH55,BM1329 and BMS2508 that were linked to the fecundity gene FecB in the sheep chromosome 6 were selected to obtain molecular markers in goat.The frequency of allele,polymorphism information content(PIC),number of effective alleles(Ne) and heterozygosity(He) of corresponding microsatellite locis in Boer goat were detected.The results indicated that there were genetic polymorphisms at these four microsatellite loci.PIC,Ne,and He at OarAE101 was 0.847 4,6.553 1,0.862 8,respectively;PIC,Ne,and He at OarHH55 was 0.767 6,4.302 9,0.796 8,respectively;PIC,Ne,He at BM1329 was 0.841 4,6.305 2,0.857 6,respectively;the PIC,Ne,He at BMS2508 was 0.783 1,4.610 4,0.809 6,respectively in Boer goats.The four microsatellite loci were height polymorphic loci which could be used for genetic polymorphism evaluation and study of the fecundity trait in Boer goats.
Key concepts: Microsatellite, Biology, Loss of heterozygosity, Genetics, Allele, Polymorphism (computer science), Gene