The Hydrolysis of Edible Gelatine and Formaldehyde-Removal Activity of It's Hydrolysate

Using amino group content as the estimated index,the condition for hydrolyzing edible gelatine with alkali protease was optimized by Box-Benhnken center combination.Then the formaldehyde-removal activity of gelatine hydrolysate was studied.The results showed as follows:(1) The optimal condition for hydrolyzing edible gelatine with alkali protease was temperature 61℃,pH 8.0,enzyme concentration 2667U·g-1 and 180min hydrolysis time.Under the optimal condition,the content of amino group was up to 0.277mol·L-1.(2) Formaldehyde could be captured by edible gelatine hydrolysate,and the formaldehyde-removal activity was significantly affected by reaction conditions,including concentrations of hydrolysate,reaction temperature,reaction time and pH.Alkaline environment could promote hydrolysate's capture of formaldehyde and there was positive correlation between the removal rate and factors such as concentration of hydrolysate,reaction temperature and time.After 1 h at 100℃,the formaldehyde-removal rate of gelatine hydrolysate were up to 51.09%,pH 7.0 and 82.77%,pH9.0,respectively.