Construction of Recombinant Adenovirus Vector Expressing the hsv-tk Gene Under the hTERT Promoter

Objective To construct the of recombinant adenovirus vector expressing the hsv-tk gene under the hTERT promoter and provide the basis for following experiments in vivo and in vitro.Methods An expression vector(PDC312-TP-TK) containing TK gene under the hTERT promoter was constructed by the homologous recombination method in bacteria.The newly recombinated PDC312-TP-TK was propagated in 293 cells and purified by cesium chloride gradient centrifugation.Positive clone was selected by using endonuclease to digest the recombinants and named PSG-TP-TK.Results The PDC312-TP-TK cDNA was confirmed by sequencing and the PSG-TP-TK was confirmed by PCR.Conclusion This investigation provides the basis for studying tumor gene therapy by PSG-TP-TK