Studies on Influential Factors of Excised Leaf Tissue Inoculation of Bacterial Leaf Streak in Oryza sativa

The excised leaf tissue inoculation(ELTI) method was used to identify the resistance of rice to bacterial leaf streak in this paper.The results showed that ELTI was a more efficient and dependable method for disease-resistance identification than the traditional method.It was necessary to keep leaf green by adding 75~100 μL/mL of benzimidazole into agar medium in the whole identification period.For efficient inoculation and identification of excised leaf tissue,the temperature was required to be 25~30 ℃,and the lowest concentration of bacterial solution should be 3×105 CFU/mL.