Increase of antioxidant activities of wheat gluten hydrolate by combined different proteases system

Based on previous study,the related conditions for obtaining higher antioxidant activity of the wheat gluten hydrolyzed with combined preteases system were well investigated in the present paper. The results showed that the combination of trypsin and neutral protease can efficiently hydrolyze wheat gluten. When ratio of trypsin to neutral protease was set as 2∶1,antioxidant activity of the hydrolate was highest. Other optimum conditions were obtained by one-factor-at-a-time and Orthogonal test design,and as follows:enzyme dosage 0.07 g,mass concentration of substrate 90 g/L,pH 9,hydrolysis temperature 50 ℃. Under the optimum conditions,the DPPH radical scavenging activities reached to 91.86% after 1.5 h hydrolysis.