Effect of Platelet-rich Plasma on the Proliferation and Osteogenic Differentiation of Human Adipose-derived Mesenchymal Stem Cells in Vitro

Objective: To evaluate the effect of platelet-rich plasma(PRP) on proliferation and osteogenic differentiation of human adipose-derived mesenchymal stem cells(hADSCs) in vitro.Methods: hADSCs were separated from an adult donor.PRP was prepared by twice centrifugation method.CaCl2 combined with thrombin was used to activate PRP.In the osteogenesis induction experiment the morphology of cells was studied with a phase contrast microscope.The alkaline phosphatase(ALP) activity of hADSCs in different concentrations PRP groups was determined by calcium-cobalt method and BCIP/NBT alkaline phosphatase color development kit was used,respectively.The proliferation of hADSCs with and without osteogenic induction culture medium in various concentrations PRP groups was detected by CCK-8 assay in 2nd,4th,6th,8th and 16th day.Results: The microscope investigation showed that 50% PRP 30μL group improved cell proliferation higher than other groups.The significant increase in ALP was observed when added original liquid PRP.The CCK-8 showed that mean A450 of original liquid PRP was higher than other groups whichever with or without osteogenic induction.Conclusion: These data suggest that PRP has a positive dose-depend and concentration-depend influence on hADSCs proliferation.