Genotoxicity of Ivermectin on Polypedates megacephalus Tadpoles

In order to assess the effect of the genotoxicity of Ivermectin( IVM) on Polypedates megacephalus tadpoles,the DNA damage in erythrocytes of the tadpoles,exposed to different concentrations of Ivermectin( 0. 000 5,0. 004 0,0. 007 5,0. 011 0,0. 014 5 mg·L- 1) for 48 h and 96 h,was investigated by the alkaline single-cell gel electrophoresis assay( SCGE). The results showed that under laboratory conditions,the DNA damage rate and arbitrary units in erythrocytes of tadpoles increased with increasing IVM concentrations and treating hours. The exposure to the concentration of 0. 000 5 mg·L- 1IVM for 48 h resulted in strongly significant increases in DNA damage rate in the tadpoles. Both of the DNA damage rate and arbitrary units were strongly significant higher than those of the controls when exposed to 0. 007 5 mg·L- 1IVM. There was strong linear correlation between arbitrary units of DNA damage and the concentrations of IVM in the same exposing time. The results of this study indicated that IVM produced DNA damage in amphibian tadpoles,and may be a risk of upsetting water ecological balance.